Technologies

Triple Vector

Phage display technology is a very powerful tool widely used to select antibody fragments such as Fabs, scFvs and VHHs. The resulting phage display selected antibody fragments are then usually converted to immunoglobulins or to Fc fusion proteins and expressed in mammalian cells. The resulting final molecules are bivalent, possess Fc effector function and a half-life comparable to natural immunoglobulins. This conversion process is a bottleneck in antibody discovery, labour intensive and time-consuming. Moreover, for some antibody fragments the conversion to Fc fusion proteins result in loss of activity. To circumvent these drawbacks, we have generated a vector (“triple vector”) containing the elements necessary to produce VHH and scFv fragments fused to human Fc in bacteria, and production in mammalian cells.

Ultimately the selection of immunological effector function bearing bivalent molecules, bypassing the cloning into mammalian expression vectors, will accelerate the drug discovery process. The selection of anti-human CXCR4 VHH-human Fc molecules from naïve repertoires using the generated triple vector is shown as case study under the “Case Studies” section.

Interested in our services?

We pride ourselves on our ability to cater to our customers' needs, please reach out to us and tell us what you need at info@iontas.co.uk

Get in touch with us
Hover to see our numbers
Species
Customers
Antibodies in Clinic
Libraries Generated
Customers
Employees
Antibody in clinic
Project Success
This site uses cookies to provide you with a great user experience. By using our website, you accept our use of cookies.